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The only 64bit TWAIN DS I am aware of currently is the sample DS on SourceForge. You can find the links at the top of http://twain.org/
On a Windows Vista64 machine I installed both the 32 and 64 versions of both the Sample application and sample ds. I also installed the older 32bit twain toolkit (twacker and ds). At this time the sample App and DS’s installers do not include the latest version of the TWAINDSM.
This machine does suffer from the DEP problem. If DEP was turned on everything failed. I updated to the latest TWAINDSM.dlls. Everything works even with DEP turned on.
I was able to scan successfully with all possible combinations of Apps to DSs.
It will require the log files to track down what is happening.
Jim Watters